Characterizations of Milkfish Sauce on Amino Acid Content with Variations in Addition of Salt and Pineapple Extract Concentration

Fish sauce is synonymous with salty taste and distinctive fish aroma. The good quality of fish sauce is supported by the amino acid content, proximate analysis and the assessment of the organoleptic preferences of the product by the panelists. This research aimed to determine the effect of increasing the concentration of pineapple extract on the organoleptic characteristics of fish sauce and the most abundant amino acid content in fish sauce and the best quality of fish sauce treatments. The research is based on randomized design of 4 days fermentation with salt : pineapple extract concentration ratio of 20%:5%, 20%:10%, 15%:15% and 25%:20% respectively. Research analysis such as amino acid content and proximate analysis were evaluated by the ANOVA test and DMRT test, while organoleptic test was evaluated by Kruskal Wallis test. The higher of the concentration of pineapple extract, the lower of amino acid content of the fish and the taste getting sourer. The best treatment for milkfish sauce was found in the treatment with salt concentration : pineapple extract = 15%:15% indicated by the highest amino acid content of glutamate, lysine and aspartate compared to other treatments, the highest protein content and water content and the best organoleptic assessment.